Università di Parma
Experimental and Applied Medical Technologies Lab


The  TECMEDLAB laboratory is fully equipped from the in-vitro to the in-vivo state-of-the-art technologies for electromechanical studies in cardiac tissue

Hereafter a brief explanation


The new multi-photon microscopy installed in 2020,  is now in place and running in our diffuse ParmaPhotonics lab!. The system is equipped with Chameleon Discovery Laser, Nikon Upright Ti Microscope,  fast-resonant scanner (15000 lines x sec.), 4 VIS detectors, 1 spectral detector.

What we can do with that: In-vivo and in-vitro Optogenetics, Calcium sparks, optical action potential, subcellular investigation of electromechanical delay, 3D reconstruction of organoids and many other projects are coming!

2) THE MUX (2014)

Mux (abbreviation for Multiplexing Epicardial Mapping) is a system capable to record 16X16 -in parallel- Electrograms signals at 8-32 KHz temporal Resolution.
Customized together with Crescent Electronics,MUX is a versatile instrument for in-vivo and ex-vivo (Langerdorff System) electrograms acquisition and stimulation for epicardial spatiotemporal mapping of impulse propagation

What we can do with that:  MUX acquire subtrheshold and supratreshold cardiac signals . Is capable to measure excitability refractoriness , conduction velocity and, due to its independent amplifiers, spatiotemporal patterns of electrical activity, isochrones, impulse propagation and arrhythmias.

3) THE Vi.Ki.E. (2016)

    ViKiE (Video Kinematic Evaluation) of cardiac contraction is a novel technology entirely developed in the Tecmedlab. Is capable to acquire tissue deformation during cardiac beating and analyzed kinematic parameters and tissue compliance.

What we can do with that: ViKIE is employed in both preclinical and clinical levels. Preclinical: estimated cardiac performance in situ or ex-vivo (Langerdorff system) and is totally combined with MUX for the acquisition of local ElectroMechanical delay. Due to the high spationtemporal resolution (pixel size 2.5 um, max fps= 1700 ) ViKiE can detect minimal tissue deformation and analyze the Energy expenditure directly linked to the ATP consumption. Clinical: ViKiE is employed in the cardiac theater (thanks to the collaboration with heart surgery in University of Verona) for measuring in real time kinematics before and after operation. To now ViKiE has been assessed for Tetrology of Fallot, CABG, Hypoplasic heart, Heart transplant and pulmonary valve replacement.

4) The LOKI (2021)

LOKI (Longitudinal OptoKinematic Incubation) is the new-entry technologies in the TECMEDLAB.  Developed in the LabVieW environment, LOKI  consists in an epifluorescence microscope equipped with three different LED sources, placed in the Incubator where an high-res videocamera is placed on the side-port.

What we can do with that: LOKI is capable to measure long.term kinematics (hours, days, months) in beating iPSC-CM, neonatal and adult cardiomyocytes. Due to its nature LOKI can do times laps video recording in both bright-field and fluorescence for  assess cell maturation, metabolism, action potential propagation, calcium transient, ATP, NOS, ROS etc. LOKI can also be employed for optogenetic and chemogenetic, thanks with the possibility to the built-in LEDs and optical fiber for light stimulation. THe final aim of LOKI is to explore several multiverses of beating cells from 2D to 3D organoids on a chip.